DNA sequencing technique

DNA sequencing technique
DNA测序技术

Medical Chinese dictionary (湘雅医学词典). 2013.

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  • DNA sequencing — ▪ genetics  technique used to determine the nucleotide sequence of DNA (deoxyribonucleic acid). The nucleotide sequence is the most fundamental level of knowledge of a gene or genome. It is the blueprint that contains the instructions for… …   Universalium

  • DNA sequencing — Procedures for determining the nucleotide sequence of a DNA fragment. Two common methods available: 1. The Maxam Gilbert technique, which uses chemicals to cleave DNA into fragments at specific bases; or, most commonly, 2. the Sanger technique… …   Glossary of Biotechnology

  • DNA-encoded chemical library — DNA encoded chemical libraries (DEL) are a new technology for the synthesis and screening of collections of chemical compounds of unprecedented size and quality. DEL represents an advance in medicinal chemistry which bridges the fields of… …   Wikipedia

  • DNA footprinting — is a method of investigating the sequence specificity of DNA binding proteins in vitro. This technique can be used to study protein DNA interactions both outside and within cells. The regulation of transcription has been studied extensively, and… …   Wikipedia

  • Sequencing by ligation — is a DNA sequencing method that uses the enzyme DNA ligase to identify the nucleotide present at a given position in a DNA sequence. Unlike most currently popular DNA sequencing methods, this method does not use a DNA polymerase to create a… …   Wikipedia

  • DNA fingerprinting — DNA fingerprint. the use of a DNA probe for the identification of an individual, as for the matching of genes from a forensic sample with those of a criminal suspect. Also called genetic fingerprinting. [1985 90] * * * Method developed by the… …   Universalium

  • DNA extraction — DNA isolation is a routine procedure to collect DNA for subsequent molecular or forensic analysis. There are three basic and two optional steps in a DNA extraction: Breaking the cells open, commonly referred to as cell disruption or cell lysis,… …   Wikipedia

  • DNA electrophoresis — is an analytical technique used to separate DNA fragments by size. An electric field forces the fragments to migrate through a gel. DNA molecules normally migrate from negative to positive potential due to the net negative charge of the phosphate …   Wikipedia

  • DNA denaturation — DNA denaturation, also called DNA melting, is the process by which double stranded deoxyribonucleic acid unwinds and separates into single stranded strands through the breaking of hydrogen bonding between the bases. Both terms are used to refer… …   Wikipedia

  • Sequencing — For the sense of sequencing used in electronic music, see the music sequencer article. For sequence learning in cognitive science, see sequence learning. In genetics and biochemistry, sequencing means to determine the primary structure (sometimes …   Wikipedia

  • DNA profiling — Not to be confused with Full genome sequencing. Forensic science …   Wikipedia

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